GENETIC DIVERSITY OF DIESEL DEGRADING BACTERIA FROM PETROLEUM CONTAMINATED SOIL OF OIL DEPOTS OF MADHYA PRADESH | Original Article
The present study envisages phylogeny of diesel degrading bacteria from oil contaminated soils of various oil filling stations of Madhya Pradesh with emphasis on the characterization of their degradation potential of diesel oil. A total of 96 bacterial isolates were isolated and identified from oil contaminated soils. The molecular characterization of all the bacterial isolates on the basis of banding patterns of ARDRA and RAPD analysis revealed that all the isolates shared significant level of genetic similarity and the differential distribution of the bacterial strains in the genotypic groups was observed. ARDRA genotypic clusters had heterogenous distribution of bacterial isolates. The analysis of degradation potential of selected ten bacterial isolates revealed that Pseudomonas aeruginosa was capable of degrading 58% of diesel within 14 days of incubation period. Fourier Transform infrared spectroscopy spectrum of degraded diesel also revealed the presence of new bands concerning to aromatic to aliphatic hydrocarbons including alcohols, ketones and nitriles. The characterization of bacterial populations living in the oil contaminated soils and evaluation of their degradation capabilities could potentially serve as guide for improving remediation of such environment.